ECG indicated a Horizontal Sloping ST Segment in Zebrafish Model. Hypoxic Cytology and Impaired Locomotion
Primary Tumor Pathology followed through Secondary Tumor Metastasis and Neovascularization
Non-invasive brain imaging
Simple convenient non-invasive brain imaging and comparative study with gross anatomy of Danio rerio
The functional brain and neuronal interactions can be closely investigated over many scales. The non-invasive brain image of zebrafish was taken using the digital camera and the gross anatomy of its brain was also imaged using the same setup. The average length scale measurement shows the active close similar brain size with (± 0.5 – 1.0 µm) variations. (Note: The deviations in measurement scale may arise due to the prolonged imaging period of the gross anatomy of brain). This method of non-invasive imaging avoids the challenging factors of time-consuming preparation of the brain intact, and the necessity of sacrificing fish. Thereby, helps in longitudinal repeated measurement and allows progressive observation of the developmental brain over the entire study period.
Human congenital hypothyroidism in Zebrafish larvae
Exogenous thyroid hormone (TH) induces premature differentiation of the zebrafish pectoral fins.
Type I Diabetes
Complete Pathophysiology of Retinopathy, Nephropathy and Neuropathy with Biochemical profiles
Human congenital Nystagmus in Zebrafish
The human congenital nystagmus is present at birth and most intently develops by 2 to 3 months of age in which the eye tends to move in a horizontal swinging fashion. These can be differentiated into two classes such as afferent nystagmus which is due to visual impairment and another is efferent nystagmus which is due to oculo-motor abnormality. The nystagmus is further divided in to two types based on oscillation such as (a) jerk nystagmus in these eye moves slowly in one direction and rapid movement in another direction (b) pendular nystagmus there is no obvious distinction between speed of movement on either direction. These zebrafish display a reverse optokinetic response that is attributed to optic nerve misrouting. The optokinetic motor response is recorded by placing the fish in a transparent plastic tube filled with methylcellulose and fix them in to glass cylinder covered with translucent screen and the eye movements are recorded by IR charge coupled camera by placing it on top of the glass cylinder and sampling was carried out for 40 frames/ second. These allows one to assess the ocular movement of the eye without killing and can be visualized in live fish.