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Zebrafish Facts

140bpm

Zebrafish has a resting heart rate of about 140 beats per minute with a similar metabolic profile as in humans. This enables a meaningful translation of data from zebrafish to humans

88%

88% of all research output in zebrafish helped to decode fundamental mechanisms of human biology in fields of  Heredity, Biomedicine, Anatomy, Neurology, Cell Biology, Molecular Biology, Physiology and Endocrinology establishing a reference for pharmacology studies

14 days

​​All most all outcomes of interventions across all therapeutic areas in zebrafish can be generated across key endpoints within 14 days

63 Models

Living and frozen libraries of zebrafish models enables immediate availability of models marrying the speed of in vitro studies and depth of in vivo studies

Zebrafish Guidelines

  • OECD 236 - In the zebrafish embryo toxicity, the freshly fertilized embryos are exposed to the test item in 5 concentrations over a course of 96 hours. Every 24 hours, four apical parameters of acute toxicity are recorded, and the lethal concentration (LC50) is calculated based on the 96 hours observation.

  • ICH 14 (FDA, 2005) - It is required that any drug candidate prior to human evaluation is tested in vitro for its effect on hERG channel expressed in cell lines and in vivo for QT prolongation. The availability of a zebrafish model with the ability to reveal QT interval-prolonging effects of compounds through multiple mechanisms could be employed.

  • ICH 2005; ICH 2015 - Each drug in development for administration to women of childbearing potential must be tested for developmental toxicity in rodent and non-rodent species. Zebrafish could be employed

  • ICH S7A - CNS studies including behavior, learning and memory, neurochemistry, optomotor, and/or electrophysiology examinations are recommended before product approval. Zebrafish could be employed.

  • ISO 10993 - This guidance document does allow testing laboratories to use new and innovative biocompatibility testing methods. The zebrafish embryo toxicity (ZET) test employs the embryos of the zebrafish (Danio rerio) to assess the toxicity of medical device polymers, drug-device combination products, and other medical device leachates.

  • ISO 12890:1999 - This International Standard specifies a semi-static method for determination of toxicity of chemicals, waters and wastewaters to embryos and early larval developmental stages of a species of freshwater fish,  Where necessary this determination may include an acute toxicity test using Danio rerio to determine the 96-h LC50 for zebrafish in accordance with ISO 7346-1, ISO 7346-2 or ISO 7346-3.