Because MASH is deeply systemic (affecting glucose metabolism, immune response, and lipid synthesis), single-target drugs are rarely sufficient. Also, many traditional models fail to mirror the full spectrum of the human disease, leading to high failure rates in clinical trials.

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A ourely diet induced model was developed to mirrorthe human condition. High fat, high carb diet fed zebrafish develops a progressive steatosis followed by lobular inflammation, ballooning and fibrosis.  Morphometric methods were used for the first level of screening

 

Morphometric Traits: Extracts specific metrics such as collagen fiber width, fiber length, and reticular network density.

 Nuclear Morphology: Calculates quantitative features like nuclear elongation, vacuolation, and texture homogeneity, tracking cellular senescence along the liver zonation

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