Hepatocellular Carcinoma

Diethylnitrosamine Induction

Diethylnitrosamine - causing DNA damage and oxidative stress

Locomotor activity, Feeding behavior, Pain, Anxiety, Social interaction

ALT, AST,Total bilirubin, CRP, VEGF

CTNNB1, PCNA, CDK4, BCL2, TNF-α, NRF2, AXIN2

Reduced hepatic metabolic capacity, Impaired detoxification function, Increased liver weight, Presence of hepatic nodules, Tumor vascularization

Hepatocyte ballooning degeneration, Cellular swelling and cytoplasmic rarefaction, Necrosis, Inflammatory cell infiltration, Microvesicular and macrovesicular steatosis, Dysplastic nodules, Abnormal vascular structures