High fat diet - causes excessive accumulation of free fatty acids, free cholesterol, and ceramides in hepatocytes
Obesity
Genotype
High cholesterol/western diet
Mechanism
High cholesterol/western diet - induces metabolic dysfunction
Phenotype
Hyperphagia, Locomotor activity, Fatigue, Anxiety, Depression, Reward behavior, Cognitive impairment, Exploratory Behavior
Biomarker
Triglycerides, Total cholesterol, LDL, HDL
Gene Expression Markers
srebp-1c, fasn, acc1, irs-1, glut4, ppar-γ, cebpα
Physiology Changes
Insulin resistance, Impaired appetite
Histology Grading Endpoint
Adipocyte hypertrophy, Fibrosis in adipose tissue, Macrophage infiltration, Hepatocyte ballooning