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"A fully Integrated
In Vivo Drug Development and Pharmacology Company"
Zebrafish is probably the most precise in vivo research model
Zebrafish larvae responds to a dose variation at picogram scale
With similar feeding behavior pattern to humans, the gut flora and endocrine system of zebrafish closely resembles human neuroendocrine system
Beyond genetic similarities with humans, zebrafish memory and cognition carry similar behavioral and physiological traits as in humans 
Key Member Profile
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Kalaichitra Periyasamy

Founder & Scientific Director

“Zebrafish generates a wide and highly sensitive phenotype response to various pathological conditions; it is inevitably the best model for simple phenotype studies to indicate if a test compound will work or not”

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Benin Joseph

Director Discovery Innovation

Zebrafish can give us credible directions into science in a very simple and quick way.

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Dr. Bibhas Kar

Chairman and Head of Scientific Advisory Comitte

“System, Standards and Supervision towards established zebrafish guidelines, building a thorough validation system for efficient use of zebrafish at Pentagrit.

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Vijayalakshmi

Animal Husbandry Manager

“ I remember more than I have done and I have done more than I remember to ensure that we replicate the natural environment of zebrafish to offer fish care and accurate stocks for research”

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Hamsavalli.M

Research Co-ordinator

The housing and experimentation on zebrafish would follow regional and international guidelines for the humane care, established standards and adherence to the 3Rs reduction, refinement and replacement

Our Technology & Capacity
Zebrafish Husbandry

  • Wild Type Zebrafish Adults & Juveniles

  • Genetic, Exogeneous Induced and Diet Model Adults, Juveniles, Larvae

  • Wild Type Larvae

  • Wide range of dosing studies in adult and larvae

  • Breeding and egg collection to meet gene manipulation studies

  • Healthy fish stocks through repeated rejuvenation of genetic lineage

Gene Manipulation

  • Microinjection World Precision Instruments, Pressurized nitrogen and Micromanipulator

  • CRISPR Cas9 based point mutation induction, homozygous, heterozygous and compound heterozygous mutation, insertional deletion, epitope segment editing for protein studies

  • Endpoint PCR and Reverse Transcriptase PCR for gene expression profiling, confirming mutation site, Sanger sequencing, PCR based assays such as sequence specific amplification, satellite marker identification

Imaging & Phenotype Measure

  • Brightfield Stereo Microscopy

  • Fluorescence Stereo Microscopy

  • Inverted Stereo Microscopy

  • Inverted Fluorescence Microscopy

  • Live Imaging & Video Processing Well Plate

  • Phenotype Capture & Video Processing – Field Test, Pain behavior, Aggression behavior, Darting motion, Stereotype behavior, Response to external stimuli

Histopathology & Immunohisto-chemistry

  • Fully facility of Histopathology for upto 3-micron sections for adults and larvae

  • Whole animal section

  • Organ specific sections

  • Pathology specific custom protocols for tissue processing

  • Quantification of tumour, cardiac hypertrophy, inflammatory infiltrate of organs, neurodegeneration and regeneration, vasculopathies, bone marrow counts, tagged large molecule localization, antibody staining, subcellular localization, lipid quantification, differential staining of infectious pathology, progress of disease quantification

  • Bone Mineral density

  • Macular degeneration and regeneration quantification

Biochemistry, ELISA, Kinetic Studies, Haematology

  • ELISA Plate Reader 10ul and 50ul sensitivity

  • Zebrafish adult and larvae euthanasia procedures and organ harvest and storage facility

  • Quantification of protein biomarkers, enzyme substrate activity profile over time, validating co factors, minerals and amino acids impact on enzyme activity

  • Quantification of biomarkers in serum, tissues and body fluids

  • Blood cell count and quantification of cell expression markers

  • Haematology smear quantification

  • Oral dose driven enzyme modulators

  • Quantification of downstream nodal protein activity amplification

  • Metabolic modulation studies

PK / PD Studies

  • Waters Alliance 2695 HPLC UV

  • Exposure Studies, Time course quantification of test compounds

  • Analysis of liver and plasma metabolites

Our Publications
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1.      Balkrishna A, Verma S, Solleti SK, Khandrika L, Varshney A. Calcio-Herbal Medicine Divya-Swasari-Vati Ameliorates SARS-CoV-2 Spike Protein-Induced Pathological Features and Inflammation in Humanized Zebrafish Model by Moderating IL-6 and TNF-α Cytokines. J Inflamm Res. 2020;13:1219-1243

2.      Balkrishna, A.; Solleti, S.K.; Verma, S.; Varshney, A. Application of Humanized Zebrafish Model in the Suppression of SARS-CoV-2 Spike Protein Induced Pathology by Tri-Herbal Medicine Coronil via Cytokine Modulation. Molecules 2020, 25, 5091

3.      Balkrishna, Acharya & Solleti, Siva & Verma, Sudeep & Varshney, Anurag. (2020). Validation of a Novel Zebrafish Model of Dengue Virus (DENV-3) Pathology Using the Pentaherbal Medicine Denguenil Vati. Biomolecules. 10. 971. 10.3390/biom10070971

4.      Balkrishna A, Rustagi Y, Bhattacharya K, Varshney A. Application of Zebrafish Model in the Suppression of Drug-Induced Cardiac Hypertrophy by Traditional Indian Medicine Yogendra Ras. Biomolecules. 2020;10(4):600. Published 2020 Apr 13. doi:10.3390/biom10040600

5.      S, Sivamani & Joseph, Joseph. J & Kar, Bibhas. (2014). Anti-inflammatory activity of Withania somnifera leaf extract in stainless steel implant induced inflammation in adult zebrafish. Journal of Genetic Engineering and Biotechnology. 12. 10.1016/j.jgeb.2014.01.002

6.      S, Sivamani & Joseph, Joseph. J & K, Nishwetha & Kar, Bibhas. (2013). Zebrafish as a model for bioavailability testing of counter drug. International Journal of Drug Development & Research. 5. 159-163

7.      S. Sivamani, B. Joseph, and B. Kar. “ZEBRAFISH: AN EMERGING MODEL SYSTEM FOR DRUG DISCOVERY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 4, July 2016, pp. 11-14, https://innovareacademics.in/journals/index.php/ajpcr/article/view/9861

8.      S, Sivamani & Kar, Bibhas. (2013). Adult zebrafish as a new animal model to study anxiety. Asian Journal of Experimental Biological Sciences. 4. 167-171

9.      S M FMB, Chitra K, Joseph B, Sundararajan R, S H. Gelidiella acerosa inhibits lung cancer proliferation. BMC Complement Altern Med. 2018;18(1):104. Published 2018 Mar 20. doi:10.1186/s12906-018-2165-1

10.  S, Sivamani & Joseph, Joseph. J & Kar, Bibhas. (2013). Zebrafish: An in vivo model for the study of liposome mediated drug delivery.. International Journal of Molecular Genetics. 3. 53-61

11.  Kar, Bibhas & S, Sivamani. (2013). Zebrafish: An in Vivo Model for the Study of Human Diseases. International Journal of Genetics & Genomics. 1. 6-11. 10.11648/j.ijgg.20130101.12

Our Location

Pentagrit

66, VOC Street, V.G.P Shanti Nagar IIT Colony, Part III, Narayanapuram, Pallikaranai,

Chennai, Tamil Nadu, India  - 600100

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