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Target Validation

Confirm Target Presence and Activity

  • Confirm drug rescue effect only in models that expresses target by using Knock Out and Knock down models generated by siRNA and CRISPR

  • Measure Target Engagement Biomarkers 

  • Track downstream markers of gene and protein expression by agonist-antagonist modulation of targets

Exploratory Toxicology

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Find out Safe Therapeutic Range

  • Know toxicity by dose range vs general systemic response of necrosis and body weight

  • By organ specific, chronic dosage, specially toxic such as reproductive, genetic, cognitive response, hERG and endocrine

  • Poor distribution profile that leads to cytokine storm driven liver and kidney toxicity, ectopic tissue, non target migration and teratogenicity

  • Vaccine hypersensitivity, off target activity and adjuvant driven

  • Drug-drug interactions and co-morbidities or secondary metabolite driven

  • OECD Fish Tests

Test No. 203: Fish, Acute Toxicity Test
Test No. 204: Fish, Prolonged Toxicity Test: 14-Day Study
Test No. 210: Fish, Early-life Stage Toxicity Test
Test No. 212: Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages
Test No. 215: Fish, Juvenile Growth Test
Test No. 230: 21-day Fish Assay : A Short-Term Screening for Oestrogenic and Androgenic Activity, and Aromatase Inhibition
Test No. 229: Fish Short Term Reproduction Assay 
Test No. 234: Fish Sexual Development Test
Test No. 236: Fish Embryo Acute Toxicity (FET) Test
Test No. 305: Bioconcentration: Flow-through Fish Test
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Based on the profile of test candidate a toxicity evaluation path can be created.

HIT Identification

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Move Superfast with Zebrafish Phenotype Rescue Screens

  • For each disease a phenotype biomarker can provide early indication of rescue potency 

  • Know impact of R group on drug potency by a quick phenotype response.

  • Evaluate different compounds under multiple dilution matrix for fragment based discovery.

  • Confirm candidates with phenotype rescue through in vivo biomarker response from tissue or fluids.

In Vivo DMPK

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Sort Kinetics Early on

  • Know if enough drug has reached the site of action through tissue distribution

  • Analyse Cmax, k, t1/2 elimination, cl, VD and AUC 

  • Corelate pharmacodynamics and toxicokinetics from various dose effect endpoints

  • Follow human routes of administration such as oral solid, oral liquid, I.P, I.V, intrathecal, intracranial, sub-cutaneous and water dissolution

  • Clarify on Drug-Drug Interactions 

 

In Vivo Pharmacology

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Generate More evidence by measuring Clinical Endpoints in Research Models

  • Screen tests compounds in research models that are genetically similar with human disease

  • Optimize lead through phenotype rescue studies

  • Validate rescue potential with pre-clinical biomarkers of clinical evaluation

  • Chart a proof of concept through detailed functional rescue assays that show dose response

  • Show comprehensive mode of action through expression analysis, biomarkers, kinetic assays

  • Build strong visual evidence through digital pathology and IHC

Translational Research

Patient Room

Predict Biomarker guided Human Dose

  • Use zebrafish dose formulas to accurately predict clinical trial dose

  • Apply translation biomarkers to guide fish data replication in humans

  •  Generate a closer drug target engagement by the use of Humanized In vivo pharmacology models provides

  • Replicate clinical endpoint scores & biomarkers assays in zebrafish

Biologics Testing

Move faster with Humanized Zebrafish with Better Expression

  • Express target proteins and evaluate safety and efficacy

  • Know if Anti Drug Antibodies are evoked

  • Generate immune cellular profiles after Vaccine Challenge Testing

  • Employ  PROTAC screening marker for Targeted Protein Degradation

Genetic Quality & Strain Testing

Make your Most Relevant Models

  • Employ CRISPR to develop the needed allelic strains 

  • Validate strains through sequencing and PCR markers

  • Confirm quality of strains through genetic tests

Xenobiotic Testing

Small Plant

Identify Safer and Effective Xenobiotics

  • Measure effect vs toxicity using zebrafish larvae screens

  • Know morphology, response toxicity, mixture toxicity confirmed through a wide range of metabolic markers

  • Know targeted impact on angiogenesis, and long term effect on thyrocytes and hepatocytes

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