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28 Myotonic Dystrophy - DM1.jpg

Multiple Sclerosis Zebrafish Model - ZFMSL

Experimental Autoimmune Encephalomyelitis (EAE) Model induced by microinjected Myelin oligodendrocyte glycoprotein (MOG) with Complete Freund's adjuvant

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Assays

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Phenotype Screening and Quantification

  1. Temperature aversion for Pain Measure

  2. Local Field Potential in Microvolts for Brain Activity Measure

  3. No of touch evokes needed per response for Numbness Measure

  4. Field Test for Distance Travelled

  5. Field Test for Time in Motion

  6. Avoidance Learning and Memory

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Relative Gene Expression profile - RT PCR

  7. CHIT1,GPNMB,CCL18,OLR1,CXCR4,CCl2, IL-6, TREM119,PLXDC2

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ELISA

  8. Nfl, MOG, sIFNAR2,MPO,CXCL1,

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Immunohistochemistry

  9. MAP2, Histological Scoring 1-5 

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