Zebrafish Model of Ulcerative Colitis ZFUCL

Phenotype Screening and Quantification

1. Intestine Length

Relative Gene Expression profile - RT PCR

  2. Cytokine Profile

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ELISA

  3. Custom

Histology

  4. Scoring and Staging

0 -No evidence of inflammation

1 - Low level of inflammation with scattered infiltrating mononuclear cells (1-2 foci)

2 - Moderate inflammation with multiple foci

3 - Severe inflammation with increased vascular density and marked wall thickening

4 - Maximal severity of inflammation with transmural leukocyte infiltration and loss of goblet cell